Journal: Scientific Reports

Article Title: Cytotoxicity and cell cycle changes in prostate cancer cells with differing PSMA expression and p53 status after treatment with PSMA-targeting radioligand [ 212 Pb]Pb-AB001

doi: 10.1038/s41598-025-29785-7

Figure Lengend Snippet: Induction of cellular senescence in PC-3 PIP cells after treatment with [ 212 Pb]Pb-AB001. Cells (0.1–1.5 × 10 6 cells per flask) were treated for 1 h with [ 212 Pb]Pb-AB001 at the indicated activity concentrations. Senescence was assessed at 1, 3, and 6 days after treatment using complementary flow cytometry and histochemical staining methods. ( a ) Fold-change in autofluorescence-corrected geometrical mean of CellEvent™ Senescence Green fluorescent signal, which detects β-galactosidase activity in live cells, relative to untreated control, measured by flow cytometry. Bars represent mean ± SD from 2–3 independent experiments, each based on acquisition of at least 10 000 events per sample. * p < 0.05, ** p < 0.01 or *** p < 0.001 versus day 1. The red dashed line indicates the untreated control. ( b ) Representative bright-field images of β-galactosidase histochemical staining for senescent cells at 1, 3, and 6 days after treatment. Increased numbers of blue-stained cells indicate treatment-induced senescence. Scalebar = 20 μm.

Article Snippet: Senescence was also detected through histochemical staining using the Senescence Cells Histochemical Staining Kit (#CS0030; Merck), as described in the manufacturer’s protocol.

Techniques: Activity Assay, Flow Cytometry, Staining, Control